Paper Publications

Peer-reviewed

Schmailzl J., Vorage M.W., Stutz H.
 Intact and middle down CIEF of commercial therapeutic monoclonal antibody products under non-denaturing conditions
Electrophoresis 41, 1109–1117 (2020)

Hinterholzer A., Stanojlovic V., Regl C., Huber C. G., Cabrele C., Schubert M.
 Identification and Quantification of Oxidation Products in Full-Length Biotherapeutic Antibodies by NMR Spectroscopy
Anal. Chem. 92(14), 9666–9673 (2020)

Paul K., Böttinger K., Mitic B. M., Scherfler G., Posch C., Behrens D., Huber C. G., Herwig C.
 Development, characterization, and application of a 2‐Compartment system to investigate the impact of pH inhomogeneities in large‐scale CHO‐based processes
Eng. Life Sci. 20(8), 368–378 (2020)

Esser-Skala W., Wohlschlager T., Regl C., Huber C. G.
 A Simple Strategy to Eliminate Hexosylation Bias in the Relative Quantification of N-Glycosylation in Biopharmaceuticals
Angew. Chem. Int. Ed. 59(37), 16225–16232 (2020)

german version:
 Eine einfache Strategie zur Korrektur des Fehlers aufgrund von Hexosylierung bei relativer Quantifizierung der N‐Glykosylierungsvarianten von Biopharmazeutika
Angew. Chem. 132(37), 16359–16367 (2020)

Di Muzio M., Wildner S., Huber S., Hauser M., Regl C., Laimer J., Vejvar E., Auzinger W., Zennaro D., Wopfner N., Huber C. G., van Ree R., Mari A., Lackner P., Ferreira F., Schubert M., Gadermaier G.
 Hydrogen/deuterium-exchange memory NMR revealed structural epitopes involved in antibody cross-reactivity of allergenic lipid transfer proteins
J. Biol. Chem. 295(51), 17398–17410 (2020)

Blöchl C., Regl C., Huber C. G., Winter P., Weiss R., Wohlschlager T.
 Towards middle-up analysis of polyclonal antibodies: subclass-specific N-glycosylation profiling of murine immunoglobulin G (IgG) by means of HPLC-MS
Sci. Rep. 10(1), 18080 (2020)

Kohlberger M., Wildner S., Regl C., Huber C. G., Gadermaier G.
 Rituximab-specific DNA aptamers are able to selectively recognize heat-treated antibodies
PLoS One 15(11), e0241560 (2020)

Without peer review

Esser-Skala W., Wohlschlager T., Huber C. G.
 In search for the needle in the Mariana Trench: host cell proteins and the problem of dynamic range
LC-GC Europe 33(S10), 13–19 (2020)

Segl M., Stutz H.
Bottom up analysis of proteins by peptide mass fingerprinting with tCITP-CZE-ESI-TOF MS after tryptic digest
Methods Mol. Biol., accepted (2020)

Participation in Conferences

Invited Talks

Wohlschlager T.
Proteinmodifikationen auf dem Prüfstand: Neue Ansätze zur Analyse von Biotherapeutika
37. Agilent Forum Analytik, Vienna (Austria), 11.–12.02.2020

Huber C.G.
Exploring the Chemical Space of Protein Glycosylation in Covalent Protein Complexes: an Expedition Along Different Structural Levels Employing Denaturing and Native Mass Spectrometry
Thermo Scientific Virtual User Meeting, online, 24.09.2020

Wohlschlager T.
Piecing together proteoforms: analytical approaches to resolve the puzzle from peptide to protein complex
Chains 2020, online, 08.12.2020

Talks

Böttinger K.
Glycosylation Profiling of Intact Monoclonal Antibodies by IP-RP HPLC-MS
30. Doktorandenseminar des Arbeitskreises Separation Science in der Fachgruppe Analytische Chemie der Gesellschaft Deutscher Chemiker, Hohenroda (Germany), 11.–14.01.2020

Participation

Di Marco F., Böttinger K.
7th Short Course EU FT-ICR MS, Biocev Center, Prague (Czech Republic), 08.12.2020

Scientific Awards and Honours

Regl C.
Merck Dissertationspreis 2019
Merck, January 2020

Esser-Skala W.
Publication of the month September
Department of Biosciences, University of Salzburg, for his paper in Angewandte Chemie (see above)

Di Muzio M.
Publication of the month December
Department of Biosciences, University of Salzburg, for her paper in Journal of Biological Chemistry (see above)

Esser-Skala W.
Publication of the year (3rd place)
Department of Biosciences, University of Salzburg, for his paper in Angewandte Chemie (see above)

Organisation of Scientific Events

37th Winterschool on Proteinases and Inhibitors
Tiers (Italy), 04.–08.03.2020

National and International Cooperations

C. Herwig
CD-Laboratory for Mechanistic and Physiological Methods for more Efficient Bioprocesses, Technical University Vienna, Austria

A. Bathke, W. Trutschnig
Statistics and Probability Research Group, Department of Mathematics, University of Salzburg, Austria

H. Lindner
Institute of Clinical Biochemistry, Biocenter, Medical University of Innsbruck, Austria

N. Kelleher
Northwestern University, Chicago, IL (USA)

S. Cianferani
Université de Strasbourg, Strasbourg (France)

O. Kohlbacher
Applied Bioinformatics Group, University of Tübingen, Tübingen (Germany)

S. Koen
Research Institute for Chromatography, Katrijk (Belgium)

S. Li
University of California, San Diego, CA (USA)

T. Zhang
Thermo Fisher Scientific, San Jose, CA (Belgium)

E. Rapp
GlyXera, Magdeburg (Germany)